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CFU per mL (Plate Count) — result sheet
Viable concentration from colonies, plated volume, and dilution.
Inputs used
Results
Visual chart
Breakdown
Calculation steps
Returned data table
Formula and methodology
Formula: CFU/mL = colonies / (volume plated x dilution factor).
Scale counted colonies back up by the plated volume and the dilution. Aim for 30-300 colonies per plate; outside that range, re-plate.
This result follows the calculator's declared inputs, precision, validation boundaries, and model limits.
Input contract
- Colonies counted — Countable plates carry 30-300 colonies.; minimum 1; maximum 1000000000
- Volume plated — mL; minimum 1.0E-6; maximum 1000
- Dilution factor — Decimal, e.g. 0.000001 for a 10^-6 tube.; minimum 1.0E-12; maximum 1
Worked example
| Input | Value |
|---|---|
| Colonies counted | 86 |
| Volume plated | 0.1 |
| Dilution factor | 1.0E-6 |
8.6e8 CFU/mL (860,000,000 CFU/mL).
Assumptions and limits
- Each colony grew from one viable cell; even spreading.
- Accurate pipetting and a decimal dilution factor.
Calculator note
Source and methodology
Use the official WorldCalculate methodology policy for the source, formula, precision, and boundary standards behind this calculator.
Planning estimate, not financial, medical, legal, or professional advice. © WorldCalculate — reuse with attribution. Built and curated by Hassan ALRowaie.
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