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Annealing Temperature Quick Screen — result sheet
Estimate a short oligo melting temperature with the educational Wallace count rule, subtract an offset for a screening annealing temperature, and report GC percentage.
Inputs used
Results
Visual chart
Breakdown
Calculation steps
Returned data table
Formula and methodology
Formula: Tm_C = 2 x (A + T) + 4 x (G + C); screening Ta_C = Tm_C - offset; GC percent = 100 x (G + C) / (A + T + G + C).
This is an explicit educational Wallace estimate from base counts for a short oligo. The total count must be 8 through 100, the offset is 0 through 15 C, and the screening temperature must remain positive. It is only a quick screen, not primer-design approval; real design tools use sequence context, salt, concentration, nearest-neighbor thermodynamics, and specificity checks.
This result follows the calculator's declared inputs, precision, validation boundaries, and model limits.
Input contract
- A count — bases; minimum 0; maximum 100
- T count — bases; minimum 0; maximum 100
- G count — bases; minimum 0; maximum 100
- C count — bases; minimum 0; maximum 100
- Screening offset — C; minimum 0; maximum 15
Worked example
| Input | Value |
|---|---|
| A count | 3 |
| T count | 2 |
| G count | 2 |
| C count | 1 |
| Screening offset | 4 |
Tm is 22 C, screening Ta is 18 C, and GC content is 37.5 percent.
Assumptions and limits
- A, T, G, and C are finite safe whole-number counts whose total length is between 8 and 100 bases.
- The Wallace estimate is Tm_C = 2 x (A + T) + 4 x (G + C), and offset is a screening adjustment from 0 through 15 C.
- The output is educational arithmetic only; sequence context, salt, concentration, nearest-neighbor thermodynamics, secondary structure, and specificity are not modeled.
Calculator note
Source and methodology
Use the official WorldCalculate methodology policy for the source, formula, precision, and boundary standards behind this calculator.
Planning estimate, not financial, medical, legal, or professional advice. © WorldCalculate — reuse with attribution. Built and curated by Hassan ALRowaie.
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